Part:BBa_K4706007

AtBAG6 induced S. cerevisae cell death

The AtBAG6 gene coding for BCL-2-associated athanogene 6 from Arabidopsis Thaliana causes when over-expressed in Plants as in S. cerevisae induced cell death phenotypes consistent with programmed cell death. The wild-type protein has a length of 1043 amino acids compared to this part which is composed of 135 amino acids. A 134-amino-acid stretch, encompassing both the IQ motif and BAG domain, was found by Kang, C. et al. to be sufficient to induce cell death.

We utilized the amino acid sequence to codon optimize the sequence for S. cerevisiae expression so no rare codons are being used.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References: 1. Kang, C., Jung, W., Kang, Y. et al. AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. Cell Death Differ 13, 84–95 (2006). https://doi.org/10.1038/sj.cdd.4401712